Points, mean value of tumor volume bars, SD. After 10 days, treatments were stopped and tumor growth was further monitored. Once the tumor reached 25 mm3 mice were randomized into four groups and treatments were started with vehicle (Control), NVP-BEZ235 (NVP, 30 mg/kg/day po), Sorafenib (Soraf, 15 mg/kg/day po), or a combination of both (NVP+Soraf). A, An equal amount of 786-0 cells were injected subcutaneously into nude mice. The relative tumor growth was further augmented when mice were treated simultaneously with NVP-BEZ235 and sorafenib (Figure 6B).Įffect of treatment cessation on tumor growth. The relative tumor growth was also significantly increased in treated mice compared to untreated mice. However, tumors significantly started to grow after 5 days without treatment (Figure 6A). We observed that the growth of 760-0 tumor xenografts was still reduced five days after drug interruption, probably reflecting residual inhibition.

At day 10, drug administration was stopped and tumor growth was monitored for an additional 10 days. To next determine the effect on tumor growth induced by the discontinuation of drug administration, nude mice bearing 786-0 cell xenografts were treated with NVP-BEZ235, sorafenib or a combination of both for 10 days. The anticancer efficacy of NVP-BEZ235 alone, or in combination with sorafenib, was also evaluated on RCC xenografts in nude mice. Tumor cell proliferation and apoptosis were investigated in vitro. The renal carcinoma cell lines 786-0 and Caki-1 were treated with NVP-BEZ235 or sorafenib, either alone or in combination.

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